M.A.G.I.C. (MFI Analysis, Graphing, Interpolation, & Calculation)

🪄 File Selection & Output Settings

Luminex CSV Data File Select your exported Luminex CSV file containing sample data

🔮 Click here to select your Luminex CSV file

Output Folder Name Name for the results folder

Output Location Choose where to save results

🪄 No custom directory selected - files will download to your default Downloads folder

✨ File Preprocessing

Configure how the Luminex CSV file should be processed and validated.

Plate Format Select the format of your Luminex plate

Number of Wells Total wells to process (auto-calculated for full plates)

🔮 Process Full Plate Process all wells in the plate (uncheck to specify custom well count)

🔮 Analysis Configuration

🔮 Optional Configuration Files

Standards Concentration File CSV with standard sample concentrations (optional - will auto-detect if not provided)

🔮 Click to select standards file (optional)

Sample Cutoff Values CSV with cutoff concentrations for sample classification (optional)

✨ Click to select cutoff file (optional)

✨ Analysis Parameters

Background Sample Name Sample name used for background subtraction

Dilution Multiplier Factor to adjust concentrations (e.g. 2 for 1:2 dilution)

Analysis Options

🪄 Detect Hook Effect Identify high-dose inhibition in immunoassays

✨ Filter Low Standards Remove standards too close to background or breaking dose-response pattern

🧙‍♂️ Plate Layout Assignment (Optional)

Import a plate layout file to assign Sample IDs to well positions. This will be matched against the processed Luminex data.

✨ Note: You can upload your plate layout file now, but full validation will be performed after the Luminex data is processed above.

Plate Layout File CSV file with Well,Sample_ID format (e.g., A1,C25C00908401)

🪄 Click to select plate layout file (optional)

🎯 Analyte Selection

Select Analyte:

⚡ Analysis Progress

Ready to begin analysis...

🌟 Results

Global Chart Axis Controls:

Use these controls to toggle all chart axes between linear and logarithmic scales.

Internal Quality Control (IQC) Analysis

This section displays data and analysis for quality control samples (IQC1, IQC2, IQC3 or Control1, Control2, Control3).

M.A.G.I.C.