CAULDRON

Curve Analysis Using Luminex Data for IC50 and neutRalisatiON

A web-based tool for analyzing neutralization assays using Luminex immunoassay data. Process dilution series to calculate IC50 values through 4-parameter logistic curve fitting.

Streamlined workflow: Upload data → Configure dilutions → Run analysis → Get IC50 results

🪄 File Selection & Output Settings

Luminex CSV Data File Select your exported Luminex CSV file containing sample data

🔮 Click here to select your Luminex CSV file

Output Folder Name Name for the results folder

Output Location Choose where to save results

🪄 No custom directory selected - files will download to your default Downloads folder

✨ File Preprocessing

Configure how the Luminex CSV file should be processed and validated.

Plate Format Select the format of your Luminex plate

Number of Wells Total wells to process (auto-calculated for full plates)

Analysis Mode Choose analysis type for your assay

Quantification Method Select quantification analysis method

🔮 Process Full Plate Process all wells in the plate (uncheck to specify custom well count)

🧪 Analysis Configuration

🔮 Dilution Series

Dilution Concentrations CSV Single row with 7-12 concentrations (e.g., 1000,500,250,125...)

📁 Upload dilution concentrations

🧬 Sample Setup

Plate Orientation How samples are arranged

Number of Samples

Background Sample Name for baseline calculation

🎯 Run Analysis

Select Analyte to Analyze Choose which analyte to process for inhibition analysis

CAULDRON

🪄 File Selection & Output Settings

✨ File Preprocessing

🧪 Analysis Configuration

🔮 Dilution Series

Loaded Concentrations:

🧬 Sample Setup

📝 Sample Name Assignment

Setup Validation:

🎯 Run Analysis

⚡ Analysis Progress

🌟 Results

Download Results